Fig. 6

SAP30 Sin3 interaction domain mediates PFV Tas deacetylation. (A) HEK293T cells were transfected with Flag-Tas and HA-P300, 3HA-SAP30 or empty vector. Forty-eight hours post-transfection, co-immunoprecipitation was performed with Flag antibodies. Western blot analysis of samples from cell lysates and immunoblotting using Flag, HA and Lys Acetylation antibodies. (B) Control and sh-SAP30 cell lines were transfected with Flag-Tas and HA-P300. Forty-eight hours post-transfection, immunoprecipitation was performed with Flag antibodies. Western blot analysis of samples from immunoprecipitates using Flag and Lys Acetylation antibodies. (C) HEK293T cells were transfected with Myc-Tas and Flag-HDAC1, 3HA-SAP30 or empty vector. Forty-eight hours post-transfection, co-immunoprecipitation was performed with Flag antibodies. Western blot analysis of samples from cell lysates and immunoprecipitates using Myc, Flag and HA antibodies. (D) HEK293T cells were transfected with Flag-Tas, HA-P300 and SAP30 truncations. Forty-eight hours post-transfection, immunoprecipitation was performed with Flag antibodies. Western blot analysis of samples from cell lysates and immunoprecipitates using Flag, HA and Lys Acetylation antibodies. (E) HEK293T cells were transfected with Flag-Tas, Flag-HDAC1 and SAP30 truncations. Forty-eight hours post-transfection, co-immunoprecipitation was performed with HA antibodies. Western blot analysis of samples from cell lysates and immunoprecipitates using Flag and HA antibodies