Fig. 5

SAP30 Sin3 interaction domain is essential for PFV transcriptional inhibition. (A) Truncations of SAP30 protein. (B–D) HT1080 cells were transfected with pcPFV and empty vector or SAP30 and different truncations. Forty-eight hours post-transfection, 600 µl of supernatants (B) or 1/10 transfected HT1080 cells (C) were co-cultured with PFVL cells. The luciferase activity was measured 48 h later, and the remaining HT1080 cells were lysed for Western blotting. Protein levels were quantified by densitometry and normalized to Tubulin levels. AU, arbitrary units (D). HT1080 cells were transfected with SAP30 or truncated plasmids and LTR-Luc (E) or IP-Luc (F) with 3.1-Tas. pCMV-β-gal was also transfected to normalize transfection efficiency. Forty-eight hours post-transfection, luciferase activities were measured and corrected by β-gal catalytic activities. The remaining cell lysates were used for Western blotting