Fig. 4

SAP30 interacts with the PFV Tas protein. (A) HEK293T cells were transfected with 3HA-SAP30 and empty vector or Flag-Tas. Forty-eight hours post-transfection, co-immunoprecipitation was performed with Flag antibodies. Western blot analysis of samples from cell lysates and immunoprecipitates using HA and Flag antibodies. (B) HEK293T cells were transfected with Flag-Tas or empty vector. Forty-eight hours post-transfection, co-immunoprecipitation was performed with Flag antibodies. Western blot analysis of samples from cell lysates and immunoprecipitates using SAP30 and Flag antibodies. (C) HeLa cells were transfected with 3HA-SAP30 or Flag-Tas or both. Forty-eight hours post-transfection, indirect immunofluorescence assay (IFA) was used to localize SAP30 (with HA antibodies and tetramethyl rhodamine isocyanate [FITC]-conjugated secondary antibodies) and Tas (with Flag antibodies and fluorescein isothiocyanate [TRITC]-conjugated secondary antibodies). Nuclei were visualized using DAPI staining