Fig. 3

SAP30 inhibits PFV transcription. (A) The level of proviral DNA integration was detected by Alu-PCR, as described in the Materials and Methods. (B and C) Co-transfection of LTR-Luc or IP-Luc with 3.1-Tas and different amounts of 3HA-SAP30 in HEK293T cells. pCMV-β-gal was also transfected to normalize transfection efficiency. Forty-eight hours post-transfection, luciferase activities were measured and corrected by β-gal catalytic activities. Remaining cell lysate was used for Western blotting. (D and E) The same experiment was repeated in HT1080 cells