Fig. 2

SAP30 inhibits PFV replication. (A–C) HT1080 cells were transfected with SAP30 or empty vector. Twenty-four hours post-transfection, cells were infected with PFV (MOI = 0.5). After 48 h, 600 µl of supernatants (A) or 1/10 infected HT1080 cells (B) were co-cultured with PFVL cells. The luciferase activity was measured 48 h later, and the remaining HT1080 cells were lysed for Western blotting (C). (D) Equal amounts of HT1080 and HeLa cells were lysed for Western blotting. (E and F) sh-Control and sh-SAP30 cells were collected, real-time PCR (E) and Western blotting (F) were performed to detect SAP30 expression levels. (G–I) Control and sh-SAP30#2 cell lines were infected with 0.5 MOI PFV. Forty-eight hours post-infection, 600 µl of supernatants (G) or 1/10 infected HT1080 cells (H) were co-cultured with PFVL cells. The luciferase activity was measured 48 h later, and the remaining HT1080 cells were lysed for Western blotting (I)