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Fig. 6 | Cell & Bioscience

Fig. 6

From: Disrupting lipid homeostasis with CAV2 in OSCC triggers apoptosis, lipolysis, and mitochondrial dysfunction by transcriptional repression of PPARγ

Fig. 6

Knockdown of CAV2 promoted lipolysis in OSCC. (A) Basal and isoproterenol-stimulated lipolysis were measured in the conditional medium of SCC25 and CAL27 cells with CAV2 knockdown. KD: shRNA targeting CAV2, labeled with GFP for green fluorescence tracking) or negative control, NC: scrambled control shRNA, labeled with GFP for green fluorescence tracking). (B) The protein expression levels of HSL, p-HSL, and PLIN3 in SCC25 and CAL27 cells after CAV2 knockdown. Quantitative analysis of HSL, p-HSL, and PLIN3 protein expression in both KD and NC groups. (C and D) The PNPLA2, LIPE, and MGLL mRNA expression in SCC25 and CAL27 cells in OSCC cells after CAV2 knockdown. (E and F) The HSL protein expression in SCC25 and CAL27 cells in OSCC cells after CAV2 knockdown. Scale bars = 50 μm. (G and H) Representative TEM of SCC25 and CAL27 cells with stable KD or NC. Scale bars = 1 μm. Frequency analysis of LD-lysosome contacts in SCC25 expressed NC (n=37) or KD (n=30) cells and CAL27 expressed NC (n=32) or KD (n=23) cells. All data were expressed as the mean ± SD (n≥3). Significance was determined by the Student’s t-test (A-F) and the Chi-square test (G and H). *P<0.05, **P<0.01, ***P<0.001, ns = no significance. CAV2: Caveolin2; OSCC: oral squamous cell carcinoma; KD: shRNA targeting CAV2, labeled with GFP for green fluorescence tracking; NC: scrambled control shRNA, labeled with GFP for green fluorescence tracking; HSL: Hormone-sensitive lipase; PLIN3: Perilipin3; TEM: transmission electron microscope

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Cell & Bioscience

ISSN: 2045-3701

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