Fig. 3

Differential chromatin accessibility and gene expression changes in Dido1∆E16 LSK cells. A. Distribution relative to transcription start sites of chromatin open in WT and closed in Dido1∆E16 LSK cells, as determined by ATAC-seq (mean values, n = 3). B. Venn diagrams illustrating the overlap in promoter regions of differentially accessible chromatin with genesets associated with PRC2 activity. C. Overlap of differentially accessible chromatin in intronic and intergenic regions. D. Validation of the expression of potentially affected genes using real-time qPCR (n = 4, t-test ** p < 0.01, * p < 0.05). E. Differential expression of Tcf3 and its two isoforms in prepro-B cells and in lin⁻ precursors, with values relative to WT prepro-B levels. F. Expression levels of various Tcf12 isoforms in Dido1∆E16 prepro-B cells, each compared to the expression of the same region in WT cells (n = 4, t-test *** p < 0.001, * p < 0.05)