Fig. 2
From: The chromatin reader Dido3 is a regulator of the gene network that controls B cell differentiation
Increased apoptosis and altered proliferation in Dido1∆E16 B cell populations compared to WT. A. Representative graphs showing annexin V binding total B220+ (left) and immature B cells (right) from the bone marrow of WT and Dido1∆E16 mice. The mean fluorescence intensities (MFI) for immature cells were 13.41 (WT, top) and 22.9 (Dido1∆E16, bottom). The lower left quadrant shows viable cells; the lower right, cells in early apoptosis (which bind annexin V and exclude DAPI); the upper right, cells in late apoptosis (which bind annexin V and incorporate DAPI); and the upper left, necrotic cells. B. Mean percentages of annexin V + cells in different precursor subpopulations (mean ± SD, n = 3, t-test * p < 0.05). C. Percentages (mean ± SD; n = 3) of cells incorporating BrdU in pro-B/pre-B and immature/mature B cell populations, from the bone marrow of WT and Dido1∆E16 mice, assessed 18 h after intraperitoneal BrdU injection