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Fig. 7 | Cell & Bioscience

Fig. 7

From: Zmynd11 is essential for neurogenesis by coordinating H3K36me3 modification of Epha2 and PI3K signaling pathway

Fig. 7

Exogenous Epha2 rescues neurogenic deficits in vivo. A Representative images of BrdU immunostaining with Ctrl, cKO + GFP and cKO + Epha2-GFP brain sections. Venus GFP and Epha2 plasmids were delivered into fetal brain via in utero electroporation at E14, and animals were sacrificed for assay at E17. BrdU was injected (100 mg/kg, i.p.) 1.5 h prior to the sacrifice. Scale bar, 50 μm. B Quantification results showed the percentage of BrdU+GFP+/GFP+ in VZ/SVZ was significantly increased in cKO + Epha2-GFP group compared to cKO-GFP group. n = 5 fetuses at least from 4 pregnant mice for each group. Data are presented as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001, one-way ANOVA analysis followed by Tukey’s multiple-comparison test, F(2, 12) = 59.67. C Representative images of PAX6 immunostaining with Ctrl, cKO + GFP and cKO + Epha2-GFP brain sections. Scale bar, 50 μm. D Quantification results showed the percentage of PAX6+GFP+/GFP+ in VZ/SVZ was significantly increased in cKO + Epha2-GFP group compared to cKO-GFP group. n = 5 fetuses at least from 4 pregnant mice for each group. Data are presented as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001, one-way ANOVA analysis followed by Tukey’s multiple-comparison test, F(2,12) = 64.34. E Representative images of Tbr2 immunostaining with Ctrl, cKO + GFP and cKO + Epha2-GFP brain sections. Scale bar, 50 μm. F Quantification results showed the percentage of Tbr2+GFP+/GFP+ in VZ/SVZ was significantly decreased in cKO + Epha2-GFP group compared to cKO-GFP group. n = 5 fetuses at least from 4 pregnant mice for each group. Data are presented as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001, one-way ANOVA analysis followed by Tukey’s multiple-comparison test, F(2, 12) = 19.6. G Representative images of Tuj1 immunostaining with Ctrl, cKO + GFP and cKO + Epha2-GFP brain sections. Scale bar, 50 μm. H Quantification results showed the percentage of Tuj1+GFP+/GFP.+ in VZ/SVZ was significantly decreased in cKO + Epha2-GFP group compared to cKO-GFP group. n = 5 fetuses at least from 4 pregnant mice for each group. Data are presented as mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001, one-way ANOVA analysis followed by Tukey’s multiple-comparison test, F(2, 12) = 11.81

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Cell & Bioscience

ISSN: 2045-3701

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