Fig. 5

Zmynd11 deficiency results in abnormal Epha2 transcription in vivo. A Immunoprecipitation (IP) followed by WB assay showed that Zmynd11 easily precipitated H3K36me3, and vice versa. Zmynd11 did not directly interact with Epha2 and RNA Polymerase II. B Schematic illustration of primers design for analyzing Epha2 promoter and DNA pull down assay. C Chromatin immunoprecipitation-qPCR (ChIP-qPCR) and quantification results showed that the enrichment of H3K36me3 at Epha2 promotor was significantly increased in cKO brain tissues compared to Ctrl. n = 3 biologically independent experiments. Data are presented as mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, unpaired Student’s t test. D ChIP-qPCR and quantification results showed that the enrichment of H3K36me3 at Epha2 promotor was significantly increased in Zmynd11 KD eNPCs compared to Ctrl. n = 3 biologically independent experiments. Data are presented as mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, unpaired Student’s t test. E ChIP-qPCR and quantification results showed that the binding of RNA Polymerase II to Epha2 promotor was significantly decreased in cKO brain tissues compared to Ctrl. n = 3 biologically independent experiments. Data are presented as mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, unpaired Student’s t test. F ChIP-qPCR and quantification results showed that the binding of RNA Polymerase II to Epha2 promotor was significantly decreased in Zmynd11 KD eNPCs compared to Ctrl. n = 3 biologically independent experiments. Data are presented as mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, unpaired Student’s t-test. G–I DNA pull down followed by WB assay and quantification results showed that the enrichment of H3K36me3 at Epha2 promotor was significantly increased, but RNA Polymerase II binding was significantly decreased in cKO brain tissues compared to Ctrl. n = 3 biologically independent experiments. Data are presented as mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, unpaired Student’s t-test