Fig. 9
From: Dysregulated ac4C modification of mRNA in a mouse model of early-stage Alzheimer’s disease
Dysregulated ac4C mRNAs identified in 3-month-old 5×FAD mice are specific to early stage of AD. A acRIP-seq performed on three independent samples from each group identified 7,189 ac4C peaks in 6-month-old WT and 8,394 peaks in 6-month-old 5×FAD, with non-overlapping regions representing peaks unique to specific samples. B A total of 3,960 ac4C-modified mRNAs were identified in at least two 6-month-old WT samples, while 4,239 were detected in 6-month-old 5×FAD samples, with all transcripts confirmed by RNA-seq. C Bar graph comparing the ratio of ac4C to non-ac4C mRNAs showed no significant difference between 6-month-old WT and 6-month-old 5×FAD mice (P > 0.99 for both comparisons). D A total of 889 ac4C mRNAs were upregulated, while 610 ac4C mRNAs were downregulated in 6-month-old 5×FAD mice. E The Venn diagram shows the overlap and distribution of ac4C mRNAs upregulated in 3 and 6-month-old 5×FAD mice compared to age-matched WT controls. A total of 134 ac4C mRNAs were upregulated in both 3-month-old and 6-month-old 5×FAD mice. In contrast, 1530 ac4C mRNAs were uniquely upregulated in 3-month-old 5×FAD mice and 755 ac4C mRNAs were uniquely upregulated in 6-month-old 5×FAD mice. F The Venn diagram shows the overlap and distribution of ac4C mRNAs downregulated in 3 and 6-month-old 5×FAD mice compared to age-matched WT controls. A total of 128 ac4C mRNAs were downregulated in both 3 and 6-month-old 5×FAD mice. In contrast, 1915 ac4C mRNA were uniquely downregulated in 3-month-old 5×FAD mice and 482 ac4C mRNA were uniquely downregulated in 6-month-old 5×FAD mice. On the basis of adjusted p value < 0.05,|Log2(FC)|> 1