Fig. 8

Reduction in protein levels of GRIN1 and MAP2 in GABAergic interneurons of 3-month-old 5×FAD mice. A-B Immunofluorescence for neurogranin (NG) and GRIN1 in the CA1 area of the hippocampus. Fluorescence intensity normalized to WT group of GRIN1 in NG-positive pyramidal neurons shows no significant difference between WT and 5×FAD mice. NS for no significant difference. C-D. Immunofluorescence for NG and MAP2 in the CA1 area of the hippocampus. Fluorescence intensity normalized to WT group of MAP2 in NG-positive pyramidal neurons shows no significant difference between WT and 5×FAD mice. E-F. Immunofluorescence for parvalbumin (PV) and GRIN1 in the CA1 area of the hippocampus, with quantification of fluorescence intensity normalized to WT group of GRIN1 in PV-positive GABAergic interneurons. A significant reduction in GRIN1 expression in PV-positive neurons is observed in the 5×FAD group compared to the WT group. *P = 0.0433. G-H. Immunofluorescence for PV and MAP2 in the CA1 area of the hippocampus, with quantification of fluorescence intensity normalized to WT group of MAP2 in PV-positive GABAergic interneurons. A significant reduction in MAP2 expression in PV-positive neurons is observed in the 5×FAD group compared to the WT group. ***P = 0.0004. All statistical analyses were performed using two-tailed t-test. Scale bars: left, 10 μm; right, 3 μm. Top panel: WT mouse; bottom panel: 5×FAD mouse. Abbreviations: so, stratum oriens; sp, stratum pyramidale; sr, stratum radiatum