A BioID-based approach uncovers the interactome of hexose-6-phosphate dehydrogenase in breast cancer cells and identifies anterior gradient protein 2 as an interacting partner

Table 1 Top 50 biotinylated proteins identified by MS after IP using streptavidin-labeled magnetic beads

Samples from parental MDA-MB-231 and stably H6PD-BirA*-HA expressing cells were incubated with 50 µM biotin for 72 h and analyzed for the presence of biotinylated proteins by label-free quantitative MS. In H6PD-BirA*-HA samples, 50 proteins were enriched by a log₂-fold change ≥ 3.33 and a p-value ≤ 0.01. PDI family members are highlighted in green, proteins with Ca²⁺-binding features are marked in blue, and endoplasmic reticulum-resident (co-)chaperones in yellow. The bait protein H6PD (bold) is marked in orange. The presented log₂- fold change represents enrichment of identified peptides in the H6PD-BirA*-HA clone samples compared to MDA-MB-231 control samples. The numbers of peptides used for quantification of each protein is listed in the “Peptides” column. Assigned gene function and subcellular localization were adapted from UniProt (24 th December 2020) and sources cited in this work. The presented data were determined based on the results from three independent experiments

ISSN: 2045-3701