Fig. 5

Co-IP of AGR2, MOGS and CANX with H6PD. MCF7 and MDA-MB-231 cells were incubated in the presence of the reversible crosslinking agents DSP and DTME, followed by immunoprecipitation (IP) using anti-H6PD antibody for pull-down and antibodies against AGR2, MOGS, CALR and CANX for the detection of potential interacting partners. β-Actin was used as negative control to exclude nonspecific binding. A representative western blot of three independent experiments is shown