Fig. 1

Clusterin expression in response to a DDC diet and bile acids. (A) Representative immunohistochemical images of clusterin expression in liver tissues of C57BL/6 mice fed a DDC diet for 10 (n = 4) and 20 days (n = 4) compared with controls (n = 4). Data in the bar graph are means ± SEM. ***p < 0.001. Scale bars indicate 100 μm. (B) Representative western blot analysis of clusterin expression in liver tissues of mice fed a DDC diet. Data in the bar graph are means ± SEM. *p < 0.05, **p < 0.01. (C) Representative western blot analysis of clusterin expression in lysates of primary HCs treated with TCA, CA, and CDCA. (D) Immunohistochemical analysis of clusterin in liver sections from patients with PBC compared with control liver tissues. Scale bars indicate 100 μm. p: portal tract