Fig. 2

CARHSP1 inhibition decreases PCa cell proliferation in vitro and in vivo. (A) Western blotting analysis of CARHSP1 protein levels in 22Rv1 and PC-3 cells transfected with CARHSP1 shRNAs (shCAR) or negative control (shCon). β-actin was used as the internal loading control. (B) Cell viability in 22Rv1 and PC-3 cells transfected with shCAR or shCon detected by an MTT assay. (C, D) Representative images of the colony formation assay in 22Rv1 and PC-3 cells transfected with shCAR or shCon and quantification analyses of the colony number. (E) Western blotting analysis of cell cycle-related proteins CDK4 and Cyclin D3 levels in 22Rv1 and PC-3 cells transfected with shCAR or shCon. β-actin was used as a loading control. (F) The growth curves of RM-1 cell xenografts in the shCon group and shCar group (n = 8 per group). Tumor volumes were measured every other day. (G) Representative image of the tumors obtained from C57BL/6 mice in the shCon group and shCar group. (H) At 14 days after cells injection, the wet weights of tumors excised from each group of mice were measured. Data are presented as means (± SD), n = 3 independent repeats. Unpaired, two-tailed t test. **p < 0.01; ***p < 0.001