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Fig. 3 | Cell & Bioscience

Fig. 3

From: The lncRNA MIR181A1HG in extracellular vesicles derived from highly metastatic colorectal cancer cells promotes liver metastasis by remodeling the extracellular matrix and recruiting myeloid-derived suppressor cells

Fig. 3

HNRNPA2B mediates the transfer of MIR181A1HG into EVs. (a) After biotin-labeled MIR181A1HG, protein samples were prepared by rapid silver staining with pull-down combined protein bands for mass spectrometry analysis and screening of potential RNA-binding proteins responsible for transportation of MIR181A1HG into EVs. (b) qPCR assays were used to assess the expression levels of MIR181A1HG in RKO- and SW620-derived EVs (b, c) and RKO and SW620 cells (d, e) upon HNRNPA2B1 knockdown. f. Schematic diagram of the three specific binding sites of HNRNPA2B1 on MIR181A1HG. WB analysis was used to examine the association between biotinylated wild-type MIR181A1HG/mutant MIR181A1HG and HNRNPA2B1 expression in samples derived by lncRNA pull-down performed with nuclear, cytoplasmic or EV lysates from RKO (g) and SW620 (h) CRC cells; biotinylated poly(G) was used as the negative control. Representative immunofluorescence images of LX2 cells cocultured with CM from RKO (i) or SW620 (j) CRC cells transfected with Cy3-MIR181A1HG (red) or si-HNRNPA2B1 are shown. (nsP>0.05, **P < 0.01)

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Cell & Bioscience

ISSN: 2045-3701

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