Fig. 2

Hepatic Enpp1-knockout exacerbates obesity and insulin resistance induced by HFD in mice. (A) Images of HFD-induced obese mice. (B) Body weight. (C) Representative images of H&E staining of mouse epididymal fat tissue and the average size of adipocytes. Scale bar, 80 μm. (D) Representative images of liver Periodic Acid-Schiff (PAS) staining and the percentage of positively stained area. Scale bar, 40 μm. (E) Blood glucose levels of mice from the indicated groups subjected to intravenous glucose tolerance test (IGTT) and the corresponding area under curve (AUC) of each group. (F) Blood glucose levels of mice in the indicated groups subjected to the insulin tolerance test (ITT) and the corresponding AUC of each group. The datas are expressed as the mean ± SEM, n = 5 per group, *p < 0.05, **p < 0.01 versus the respective controls