Fig. 1

Establishment of MASH-HCC disease progression in DIAMOND mouse model. Wild-type mice with mixed backgrounds of C57BL/6J and 129S1/SvlmJ (male, 21–24 weeks old) were fed with WDSW or CDNW for 6 months (6 M) or 1 year (1Y). (A) Representative liver images. (B) Representative images of the liver sections with H&E staining. (C) Serum levels of liver enzyme levels (ALT, AST, ALP), and total CHOL. Data are presented as the mean ± SEM. Statistical significance relative to the corresponding CDNW group: *p < 0.05, **p < 0.01, ***p < 0.001. Statistical significance relative to WDSW-6 M group: ##p < 0.01 (n = 10–15). WDSW: Western Diet and Sugar Water; CDNW: Control Diet and Normal Water; ALT: Alanine Aminotransferase; AST: Aspartate Aminotransferase; ALP: Alkaline Phosphatase; CHOL: Total Cholesterol