Fig. 8
SEPP1 recombinant protein enhances the efficacy of anti-PD-1 therapy in PC mouse model. KPC and Pan02 cells (5 × 105 cells) were injected into the flank of C57BL/6 mice subcutaneously. (A) The scheme of the in vivo experiment design. (B) Representative photos of xenograft tumors and tumor volumes in normal mouse xenograft model. Pan02 cells (1 × 106 cells) were injected into the tail vein of C57BL/6 mice. (C) The proportion of CD8+/IFN-γ+ T cells was analyzed by flow cytometry. (D) The killing efficacy of CD8+ T cells was detected by CTL. (E) The number of MDSCs was analyzed by flow cytometry. *, P < 0.05; **, P < 0.01; ***, P < 0.001. n = 5