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Fig. 2 | Cell & Bioscience

Fig. 2

From: CircRNA Itm2b induces oxidative stress via the interaction with Sirt1-Nox4 to aggravate sleep disturbances after traumatic brain injury

Fig. 2

Increased Nox4 level, losses of rhythm proteins, and sleep architecture disturbance in TBI patients. A Relative expression of hsa_circ_0006620 in the brain specimens from acute TBI patients, TBI patients n = 6, controls n = 3. TBI vs. control, **** p < 0.0001. B, C Representative images of double immunofluorescence show the co-localization of NOX4, CLOCK, and BMAL1 in patients with acute TBI. D The expressions of NOX4, CLOCK, and BMAL1 are detected by western blotting in TBI patients. TBI patients n = 6, controls n = 3. NOX4: TBI vs. Control group, **** p < 0.0001; CLOCK: TBI vs. Control group, *** p < 0.001; BMAL1: TBI vs. Control group, *** p < 0.001. One-way ANOVA followed by Tukey’s multiple comparisons test. E Representative EEG waves of normal, mild TBI, and moderate TBI groups. Mild TBI group, n = 28; moderate TBI group, n = 22; normal group, n = 20. TBI. Patients underwent EEG recordings 30 days after TBI. Note: In the normal group, no injury was present on either side, serving as a baseline for comparison. For statistical analysis, the corresponding side of the normal group was used to compare with the injured side of the experimental groups. F The nest histogram shows the percentage of each wave energy (alpha, beta, theta, and delta) relative to the total energy in sleep duration in both the ipsilesional and contralesional cortex at 30d in TBI patients in different groups. Mild TBI group, n=28; moderate TBI group, n=22; normal group, n=20. Ipsilesional cortex (left panel): Mild TBI delta vs. Normal group delta, **** p<0.0001; moderate TBI delta vs. Mild TBI delta, ### p<0.001; contralesional cortex (middle): Mild TBI delta vs. Normal group delta,**** p<0.0001; Moderate TBI delta vs. Mild TBI delta, ns not statistically significant, one-way ANOVA followed by Tukey’s multiple comparisons test. The correlation analysis (right panel) between delta energy density in sleep duration and expression of hsa_circ_0006620 in the serum of TBI patients are shown. Grey points and lines represent the normal group, normal group correlation: p=ns (not statistically significant); green points and lines represent the mild TBI group (GCS 13-15), mild TBI group correlation: p=ns (not statistically significant); red points and lines represent the moderate TBI group (GCS 8-12), moderate TBI group correlation: p=0.0347. The delta energy density in sleep duration negatively correlated with the expression level of hsa_circ_0006620 in the moderate TBI group. All data were represented as mean ± SD

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Cell & Bioscience

ISSN: 2045-3701

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