Fig. 2

Spatial immune profiles of healthy livers and MASH-HCC treated with or without miR-22. A Spatial immune profiling of CD45⁺ cells, including macrophage, dendritic cells (cDC1, cDC2, moDCs, pDCs), T cells (Treg, CD4 Tm, Naïve CD8, Tgd, NK T), B cells, and plasma cells in the tumor, at the tumor margin, and outside the tumor of MASH-HCC mice. # indicates statistical significance based on location comparisons between margin (M) vs. tumor (T) or outside tumor (OT) vs. tumor (T). B Changes in immune cell types, including neutrophils, basophils, eosinophils, monocytes, T cells, and B cells, in the tumor of MASH-HCC in response to miR-22 treatment. C Changes in specific immune cell subtypes at the margin regions of MASH-HCC following miR-22 treatment. Data are presented as mean ± SD, analyzed using ANOVA with Tukey's test. * or # p = 0.05, ** or ## p = 0.01, *** or ### p = 0.001, where # indicates comparisons between MASH-HCC vs. healthy liver and * indicates miR-22 treated vs. untreated MASH-HCC