Fig. 6
From: Plectin, a novel regulator in migration, invasion and adhesion of ovarian cancer
PLEC promoted ovarian cancer cell adhesion in vitro with COL17A1 and ITGβ4. A The image fragmented cell monolayers which were detached with dispase and mechanically fragmented by pipetting. B The area of those cell fragments quantification by imageJ, error bars represent. C pre-coating the 96-well plate with the coating solution from the adhesion detection kit, seeding the cells in the 96-well plate at a density of 5 × 104 cells per well, adding 10 µl of cell staining solution to each well for 2 h after incubation, and subsequently measuring the OD value of the sample wells after 4 h, followed by statistical analysis of the data. D COL17A1 and ITGβ4 are highly related with PLEC (https://cn.string-db.org/). E Confocal assays (green: PLEC, red: ITGβ4/COL17A1, blue: DAPI). F IHC of tumor slides. G The result of statistical analysis of the fluorescence intensity value expressed by PLEC, ITGβ4 and COL17A1 in B, quantified by imageJ, error bars represent SD. P-value: *<0.05; **<0.01; ***<0.001; ****<0.0001, determined by two-tailed Student’s t-test