Fig. 3
From: Plectin, a novel regulator in migration, invasion and adhesion of ovarian cancer
PLEC promoted ovarian cancer cell proliferation both in vitro and in vivo.A Colony formation assays of A2780 and SKOV3 PLEC knock-down and control EOC cells. Cells were fixed 2 weeks later, stained with crystal violet. B The result of statistical analysis of the area covered by colonies across average expressed by PLEC in A, quantified by imageJ, error bars represent SD. C Cells were seeded into 96-well plates at 5 × 103/well, The OD value of 450 nm was determined at 24/48 h. Error bars represent SD. D Cells were seeded into 96-well plates at 4 × 103/well, count the cell number at 24/48 h. E Injected the mixture of A2780 PLEC knock-down and control cells (1 × 108/ml) and matrigel matrix subcutaneously into the BALB/c nude mice (n = 5) till the tumor volume reached tissues approximately 1000–2000 mm3. F Excised the tumors from the mice. G Tumor volume was measured every five days with vernier calipers, error bars represent SD. P-value: *<0.05; **<0.01; ***<0.001; ns: no significance, determined by two-tailed Student’s t-test