Fig. 4

Characterizations of NO and BO after RSV. (A). Stepwise protocol for generating organoids infected with RSV. (B) Representative light microscopy images of infected NO and BO. Scale bar: 50 μm. (C) Quantification of RSV-N gene copies in NO and BO at 24 h post infection (hpi) and 48 hpi. * p < 0.05; ns: not significant; n = 4 replicates. (D) Immunofluorescence analysis of RSV-N protein expression levels. Scale bar: 50 μm