Fig. 3

Results of whole-exome sequencing (WES) and RNA sequencing (RNA-seq) in NO and BO. (A). Venn diagrams of WES between NO and BO. Venn diagrams depicting the overlap and unique genetic variations identified in the NO and BO through WES. (B). Venn diagrams of RNA-seq data between NO and BO. (C). Heatmap showing the differential expression profiles of genes between the two study groups and the homogeneity in each group according to RNA-seq. Each row represents a gene, and each column represents an organoid sample. The color gradient indicates the level of gene expression, providing a visual representation of the transcriptional differences. This protein regulates epithelial cell proliferation and protein O-linked glycosylation and adaptive and innate immune response genes between NO and BO. (D and E) KEGG enrichment analysis of upregulated genes (E) and downregulated genes (D) in NO compared with BO