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Fig. 2 | Cell & Bioscience

Fig. 2

From: Ancestral retrovirus envelope protein ERVWE1 upregulates circ_0001810, a potential biomarker for schizophrenia, and induces neuronal mitochondrial dysfunction via activating AK2

Fig. 2

Characteristics of circ_0001810 in SHSY-5Y cells. A Design of convergent and divergent primers for circ_0001810 detection. B Amplification of circ_0001810 and GAPDH from cDNA and gDNA of SHSY-5Y cells using divergent and convergent primers, respectively. C RT-qPCR analysis of circ_0001810, NCOA2, and GAPDH mRNA levels in SHSY-5Y cells with or without RNase R treatment. D Stability of circ_0001810 and NCOA2 mRNA in SHSY-5Y cells treated with Act D assessed by RT-qPCR. E Subcellular localization of circ_0001810 in SHSY-5Y cells, with GAPDH and U6 as controls. F Detailed construction of the circ_0001810 overexpression vector. G Detection of circ_0001810 levels following its overexpression in SHSY-5Y cells and primary neurons. H The mRNA expression of NCOA2 in SHSY-5Y cells post circ_0001810 overexpression. Each bar represented the mean ± SD of three independent experiments. ns p > 0.05, * p < 0.05, ** p < 0.01

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Cell & Bioscience

ISSN: 2045-3701

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