Fig. 1

Hepatic MCU expression is upregulated in patients with MASLD and MASH. A Representative H&E and Masson’s trichrome staining in liver sections (scale bars: 200 μm). B Representative MCU immunostaining in liver sections from our cohort of MASLD/MASH patients. Bottom row (scale bars: 100 μm) contains images enlarged from the boxed area in the corresponding panel in the top row (scale bars: 200 μm). C Areas of MCU⁺ cells (%) per field (n = 11–13/group) were analyzed by ImageJ. D Database-based gene expression analysis was conducted using public data sets obtained from GEO at site the NCBI (http://www.ncbi.nlm.nih.gov/geo/). The data were processed as median-normalized signal intensity values. Healthy control (n = 24), simple steatosis (n = 20) and MASH (n = 19) for GEO: GSE89632