Fig. 7

PDE inhibition upregulates tyrosine hydroxylase Ser40 phosphorylation in the mouse striatum. Quantitative analysis of the effects of the non-selective PDE inhibitor IBMX (A), different concentrations and/or times of exposure to the PDE2A inhibitor BAY 60-7550 (B–F), or the PDE2A inhibitor PF05180999 (G–I) on tyrosine hydroxylase Ser40 phosphorylation in mouse striatal slices. A Exposure to the non-selective PDE inhibitor IBMX (10 µM) for 60 min upregulates tyrosine hydroxylase Ser40 phosphorylation in mouse striatal slices (n = 11). B–F Exposure to the PDE2A inhibitor BAY 60-7550 for 60 min at concentrations of 0.1 µM (B; n = 10) and 1 µM (C; n = 11) have no effect on Ser40 phosphorylation, as well as after 30 min at a concentration of 10 µM (D; n = 5). However, after 60 min exposure at concentrations of 10 µM (E; n = 10) and 100 µM (F; n = 10), BAY 60-7550 upregulates tyrosine hydroxylase Ser40 phosphorylation in mouse striatal slices. G–I Exposure to the PDE2A inhibitor PF05180999 (10 µM) for 15 min had no effect (G; n = 5) on tyrosine hydroxylase Ser40 phosphorylation in mouse striatal slices, while it upregulates Ser40 phosphorylation after both 30 (H; n = 5) and 120 (I; n = 11) minutes of exposure to PF05180999