Fig. 3

Tanycytes lining the lateral and ventral wall of the third ventricle (3 V). A Classical distribution of tanycytes in the tuberal region of the hypothalamus (adapted with the permission of Prof. Fanny Langlet). B The sagittal schematic showing zone 1 (from bregma – 1.3 to – 1.6 mm), zone2 (from bregma – 1.6 to – 1.8 mm), zone 3 (from bregma – 1.8 to – 2.1 mm) and zone 4 (from bregma – 2.1 to – 2.5 mm) along the anteroposterior axis. C Immunohistochemistry for the intermediate filament vimentin (white) showing all subtypes of tanycytes and ependymocytes populating the 3 V wall of mice. The tanycytic layer (distance from the 3 V bottom to the top tanycytes) and the 3 V surface (distance from the bottom to the top of 3 V) are delineated. Scale bar, 300 μm. D Vimentin-positive tanycytes distribute from zone 1 to zone 4 in the old group. Pattern of tight junction protein ZO-1 (red) is honeycombed in bottom 3 V of the old group. E The ratio of 3 V surface occupied by tanycytic layer revealed no differences in tanycyte territory between old groups of WT and AD mice (n = 5). Two-way ANOVA with Bonferroni’s post hoc test