Fig. 1
Comparative analysis of amyloid plaques in hypothalamic subdivisions. A Nucleus staining (Hoechst, blue) reveals four regions of hypothalamus in mice, including preoptic, anterior, tuberal, and mammillary regions. Nuclei indicated by dashed circles are labeled on the other side of the same slice. AC anterior commissure, MPO medial preoptic nucleus, LPO lateral preoptic nucleus, PVN paraventricular nucleus, AHC anterior hypothalamic nucleus, SCN suprachiasmatic nucleus, SON supraoptic nucleus, LH lateral hypothalamus, DMH dorsalmedial nucleus, VMH ventralmedial nucleus, ARH arcuate nucleus, TuN tuberal nucleus PH posterior hypothalamic nucleus, SuM supramammillary nucleus, MM mammillary bodies. Scale bar: 1 mm. B Representative images of 6E10 and Aβ1–42 co-staining in hypothalamic preoptic and anterior regions of Old-AD-Male and Old-AD-Female subsets. Corresponding quantification of 6E10 +and Aβ1–42 +load (n = 5–10). C Representative images of 6E10 and Aβ1–42 co-staining in hypothalamic tuberal and mammillary regions of Old-AD-Male and Old-AD-Female subsets. Corresponding quantification of 6E10 +and Aβ1–42 +load (n = 5–10). *p < 0.05; **p < 0.01; ***p < 0.001, two-way ANOVA followed by Bonferroni’s multiple comparison