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Fig. 4 | Cell & Bioscience

Fig. 4

From: Reduction of eEF2 kinase alleviates the learning and memory impairment caused by acrylamide

Fig. 4

eEF2K-KO mice can improve behavioral performance related to learning and memory impairment caused by ACR exposure. (A) Flowchart of experiments conducted on eEF2K-KO mice. (B) Body weight changes in eEF2K-KO mice exposed to ACR for 28 days. (C) Immunohistochemistry was used to observe the expression of eEF2K in the DG area of hippocampal tissue. Blue is the nucleus, and brown or tan is eEF2K. Black arrows indicate the positive expression of eEF2K. Take pictures under a 200× light microscope, scale bar: 100 μm. (D) Expression of eEF2K-positive cells in the DG area of hippocampal tissue. Data are expressed as means ± SD, n = 3. ****P < 0.0001. (E) Effects of ACR on eEF2, eEF2K, and p-eEF2 protein expression in eEF2K-KO mice. (F) The protein expression intensity of eEF2K in the hippocampus tissue of eEF2K-KO mice. Data are expressed as means ± SD, n = 3. *P < 0.05. (G) The protein expression intensity of p-eEF2/eEF2 in the hippocampus tissue of eEF2K-KO mice. Data are expressed as means ± SD, n = 3. ****P < 0.0001. (H) Path diagram and heat map of open field testing. (I) Peripheral grid movement time in the open field test. Data are expressed as means ± SD, n = 6. *P < 0.05, ****P < 0.0001. (J) Centre grid resting time in the open field test. Data are expressed as means ± SD, n = 6. ***P < 0.001; ****P < 0.0001. (K) Percentage of unconditioned responses in shuttle box test. Data are expressed as means ± SD, n = 6. *P < 0.05; **P < 0.01. (L) Percentage of conditioned responses in shuttle box test. Data are expressed as means ± SD, n = 6. *P < 0.05; **P < 0.01

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