Your privacy, your choice

We use essential cookies to make sure the site can function. We also use optional cookies for advertising, personalisation of content, usage analysis, and social media.

By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some third parties are outside of the European Economic Area, with varying standards of data protection.

See our privacy policy for more information on the use of your personal data.

for further information and to change your choices.
Skip to main content
Fig. 3 | Cell & Bioscience

Fig. 3

From: Generation and transcriptomic characterization of MIR137 knockout miniature pig model for neurodevelopmental disorders

Fig. 3

Comparative analyses of miR-137 deficiency in mouse, pig and hiPSC-derived neuron models. A Generation of the Mir137 knockout mice. A targeting vector was designed to disrupt the Mir137 gene via homologous recombination in mouse embryonic stem cells, with two loxP sites inserted upstream (~ 2 kb) and downstream (~ 0.6 kb) of the Mir137 gene (top). Crossing with Zp3-Cre mice led to the specific deletion of Mir137 in the germline, resulting in the generation of homozygous Mir137 knockout (Mir137–/–) mice (bottom). B Generation of MIR137 knockout hiPSC-derived forebrain neuron. Specific sgRNAs were designed and constructed to target the human MIR137 gene. The CRISPR/Cas9 gene editing technique was used to deliver Cas9 and sgRNAs into hiPSC via electroporation. Monoclonal cells with CRISPR/Cas9-induced MIR137 deletion (MIR137–/–) were isolated and utilized to generate neural progenitor cells (NPCs) and forebrain neurons. C qPCR verification of decreased expression of mature miR-137 in both MIR137–/– hiPSC-derived NPCs and forebrain neurons. Data are presented as means ± SEM. Statistical significance was tested with unpaired t-test, with ***p < 0.001 and ****p < 0.0001. D Number and percentage of DEGs in pig, mouse and hiPSC-derived forebrain neurons. Mouse and pig gene symbols were converted to human gene symbols using Ensembl Biomart for cross-species comparison. Genes that could not be converted were excluded from the analysis. E–F Top 5 GO terms, enriched pathways and disease associations for DEGs identified in Mir137–/– mouse brain (E) and MIR137–/– hiPSC-derived forebrain neurons (F). G Heatmap showing gene expression of neuronal marker genes across species. Neuronal marker genes overlapping with DEGs identified in MIR137–/– miniature pig was included. Marker genes were sourced from a public database (http://xteam.xbio.top/CellMarker/). H UpSet diagram showing the intersections of DEGs in miniature pig, mouse, hiPSC-derived forebrain neurons, and genes associated with autism spectrum disorder (ASD), intellectual disability (ID), and schizophrenia (SCZ)

Back to article page

Cell & Bioscience

ISSN: 2045-3701

Contact us