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Fig. 1 | Cell & Bioscience

Fig. 1

From: Generation and transcriptomic characterization of MIR137 knockout miniature pig model for neurodevelopmental disorders

Fig. 1

Generation of MIR137 knockout (MIR137–/–) miniature pigs. A Experimental procedures for MIR137–/– miniature pig model generation. PFF, porcine fetal fibroblast. B Schematic diagram of sgRNA-targeting sites in the porcine MIR137 gene. C Summary of embryo transfer data from somatic cell nuclear transplantation (SCNT) of the MIR137–/– embryo to generate mutant miniature pig models. D Representative image of a newborn MIR137–/– piglet. E PCR genotyping results of the MIR137–/– miniature pigs. F Genomic coverage of PCR-free whole-genome sequencing (WGS). G PCR-free WGS analysis revealed a 74-bp deletion of MIR137 in the genome. H Quantitative RT-PCR analysis of mature miR-137 expression levels in MIR137–/– miniature pigs and wild-type (WT) control miniature pigs. Data are represented as mean ± SEM. Statistical significance was tested with unpaired two-tailed t tests, with ****p < 0.0001

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Cell & Bioscience

ISSN: 2045-3701

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