Fig. 5

Inducible deletion of PRMT1 in the intestinal epithelium dramatically increases enteroendocrine cells (EECs) in adult small intestine. A Immunofluorescent staining for CHGA in small intestinal cross-sections from PRMT1^indΔIEC and PRMT1^fl/fl littermates at day 14 after the first tamoxifen injection as diagramed in Fig. 1A. The CHGA-labeling stained EEC cells red as indicated by arrows, and the DNA was stained blue with DAPI. B Quantification of CHGA + cells showed that PRMT1 deletion in adult intestinal epithelium dramatically increased the number of EECs. Multiple sections per animal were analyzed for each group. The values were presented as mean ± SEM with n = 3–4 mice per group. ***p < 0.001. Scale bars; 100 μm. CHGA: Chromogranin. C Immunofluorescent staining for serotonin in small intestinal cross-sections from PRMT1^indΔIEC and PRMT1^fl/fl littermates at day 14 after the first tamoxifen injection as diagramed in Fig. 1A. The serotonin-positive cells were stained green as indicated by arrows, and the DNA was stained blue with DAPI. D Quantification of serotonin-positive cells showed that PRMT1 deletion in adult intestinal epithelium increased the number of serotonin-positive cells, the most abundant subtype of EECs. Multiple sections per animal were analyzed for each group. The values were presented as mean ± SEM with n = 3–4 mice per group. ****p < 0.0001. Scale bars; 100 μm