Synthetic β-sheets mimicking fibrillar and oligomeric structures for evaluation of spectral X-ray scattering technique for biomarker quantification

Table 3 Summary of parameters extracted by analyzing ATR-FTIR and solution SAXS spectra of α-synuclein fibril, Aβ-42 fibril and modified and un-modified protein solutions of LGAB and BSA

Sample	Vibrational frequency peak center of β-sheet/ aggregated structure (cm⁻¹)	% β-sheet	Particle shape	\({R}_{g}\)(nm)	a (nm)	b (nm)
α- synuclein fibril- pH 7.4	1630	51 ± 2	Elliptical cylinder		3.5 ± 0.05	13.3 ± 0.2
Aβ-42 fibril-pH 7	1626	55 ± 2	Elliptical cylinder		3.0 ± 0.08	9.2 ± 0.1
LGAB fibril-pH 2	1624	54 ± 3	Elliptical cylinder		1.1 ± 0.03	9.8 ± 0.09
BSA fibril-pH 2	1617	33 ± 5	Elliptical cylinder		1.4 ± 0.2	10.4 ± 0.4
LGAB-pH 2	1628	54 ± 2	Prolate ellipsoid	2.3 ± 0.05	1.5 ± 0.01	3.2 ± 0.02
LGAB-pH 7	1628	55 ± 4	Prolate ellipsoid	2.2 ± 0.01	1.3 ± 0.05	3.3 ± 0.04
BSA-pH 2	1622	20 ± 3	Prolate ellipsoid	3.0 ± 0.02	1.3 ± 0.02	4.6 ± 0.02
BSA-pH 7	1622	18 ± 2	Prolate ellipsoid	2.9 ± 0.01	1.4 ± 0.01	4.5 ± 0.02

Averaged values ± standard deviations are from three independent measurements on fresh samples

ISSN: 2045-3701