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Fig. 1 | Cell & Bioscience

Fig. 1

From: Epithelium-specific Ets transcription factor-1 acts as a negative regulator of cyclooxygenase-2 in human rheumatoid arthritis synovial fibroblasts

Fig. 1

ESE-1 and COX-2 are induced by IL-1β in human RASFs. a Changes in ESE-1 and COX-2 transcriptional levels in human patient RASFs (n = 5) during IL-1β (10 ng/mL) stimulation by quantitative RT-PCR normalized to GAPDH. b Quantification of prostaglandin E2 (PGE2) in the culture media of human RASFs (n = 6) stimulated with 10 ng/mL of IL-1β for the designated time points. c Representative Western blot from four independent patients showing changes in ESE-1 and COX-2 at protein level, using Hsp90 as a loading control. Bars show mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001

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Cell & Bioscience

ISSN: 2045-3701

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