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Table 1 Small molecule probes for H 2 O 2 imaging

From: Recent advances in hydrogen peroxide imaging for biological applications

Fluorescent probes

Excitation/Emission (nm)

Additional features

References

Abbreviation

Full name

 

Detection mechanism

Intra-cellular

In vivo

 

PX-1

Peroxyxanthone-1

350/440

Boronate-deprotection mechanism

Yes

N/A

[31]

FP-H2O2-NO

N/A

400/460

Response to H2O2, NO, or both

Yes

N/A

[32]

DMACA

N/A

400/484, 566

Ratiometric imaging

Yes

N/A

[33]

DPPEA-HC

7-hydroxy-2-oxo-N-(2-(diphenylphosphino)ethyl)-2H-chromene-3-carboxamide

403/449

PET control

N/A

N/A

[34]

PL-1

Peroxy Lucifer 1

410/475, 540

Boronate-deprotection mechanism; Ratiometric imaging

Yes

N/A

[35]

RPF-1

Ratio Peroxyfluor 1

420/464, 517

Boronate-deprotection mechanism; Ratiometric imaging

N/A

N/A

[36]

PF-1

Peroxyfluor-1

450/>460

Boronate-deprotection mechanism

Yes

N/A

[31, 37]

PF-2

Peroxyfluor-2

475/511

Boronate-deprotection mechanism

Yes

Yes

[25, 38, 39]

PF6-AM

Peroxyfluor-6 acetoxymethyl ester

482/517

Boronate-deprotection mechanism; High cellular permeability

Yes

N/A

[2, 25, 28]

NBzF

N/A

490/525

PET control

Yes

N/A

[40]

PF-3

Peroxyfluor-3

492/515

Boronate-deprotection mechanism

Yes

N/A

[38]

RF-1

Redoxfluor 1

495/503

Boronate-deprotection mechanism

Yes

N/A

[41]

MitoPY1

Mitochondrial Peroxy Yellow 1

510/528

Boronate-deprotection mechanism; Mitochondria-targeted

Yes

N/A

[25, 42, 43]

PY1-ME

Peroxy Yellow 1 Methyl-Ester

515/540

Boronate-deprotection mechanism

Yes

N/A

[44]

(NucPE) 1

Nuclear Peroxy Emerald 1

514/540

Boronate-deprotection mechanism; Nuclear localized

Yes

Yes

[45]

PY1

Peroxy Yellow 1

519/548

Boronate-deprotection mechanism

Yes

N/A

[25, 38]

PO1

Peroxy Orange 1

540/565

Boronate-deprotection mechanism

Yes

N/A

[25, 38]

Amplex Red

Amplex Red

571/581

Conjunction with horseradish peroxidase

N/A

Yes

[1, 46]

PR-1

Peroxy Red 1

~575/585

Boronate-deprotection mechanism

Yes

N/A

[31]

PCL-1

Peroxy Caged Luciferin 1

NA/612

Boronate-deprotection mechanism; Bioluminescence imaging

N/A

Yes

[30, 47]

PCL-2

Peroxy Caged Luciferin 2

 

Boronate-deprotection mechanism; Bioluminescence imaging

N/A

Yes

[48]

QCy7

Quinone Cy-7

595/635

Cyanine-based probe

N/A

Yes

[49]

PN1

Peroxy Naphthalene 1

TPM750nm

Boronate-deprotection mechanism

N/A

Yes

[50]

SHP-Mito

N/A

TPM750nm

Boronate-deprotection mechanism Mitochondria-targeted

Yes

Yes

[51]

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Cell & Bioscience

ISSN: 2045-3701

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