Figure 8

Oxidative Stress Test. (A) phase contrast, (B) LAMP-2 and (C) LipidTOX staining of 10 mM hydrogen peroxide-treated ARPE-19. (D) Overlay showing co-localization of neutral lipid and LAMP-2. There are no differences in cell cytoplasm (vacuoles, lipids) in phase or fluorescence between control (Fig 2 A-D) and hydrogen peroxide treatment.